《植物生理学报》 2016, 52(8): 1199-1206

盐胁迫下巴西蕉幼苗部分细胞膜系统Ca²⁺-ATPase的活性变化

王文昌^1,2, 乔飞², 江雪飞¹, 周双云³, 吉福桑¹, 李新国^1,*

¹海南大学园艺园林学院, 海口570228; ²中国热带农业科学院热带作物品种资源所, 海南儋州571737; ³广西农业科学院园艺研究所, 南宁530007

通信作者：李新国；E-mail: lixinguo13@163.com

摘　要：

为研究盐胁迫下微粒体膜Ca²⁺-ATPase活性变化, 试验采用营养液培养的方法, 研究巴西蕉幼苗在60 mmol•L^-1的NaCl胁迫4、8、12、24和48 h下, 增加外源CaCl₂和钙离子螯合剂EGTA处理, 测定其叶片和根部分细胞膜上的Ca²⁺-ATPase活性。结果表明, 巴西蕉幼苗根和叶片的质膜、液泡膜及内质网膜的Ca²⁺-ATPase活性各不同, 且CaCl₂处理的Ca²⁺-ATPase活性均显著高于其它3种处理。在NaCl胁迫4 h时, CaCl₂处理的Ca²⁺-ATPase活性均显著升高, 而EGTA处理的则显著下降。幼苗质膜和液泡膜的Ca²⁺-ATPase活性远远大于内质网膜Ca²⁺-ATPase。这表明了质膜和液泡膜Ca²⁺-ATPase活性主要参与巴西蕉幼苗Ca²⁺稳态的调控。

关键词：巴西蕉幼苗; 盐胁迫; Ca²⁺-ATPase活性

收稿：2016-03-07   修定：2016-06-06

资助：国家自然科学基金(31260462)和海南大学中西部计划学科建设项目(ZXBJH-XK008)。

Changes of Ca²⁺-ATPase activities of part of cell membrane system in Brazilian banana seedling under salt stress

WANG Wen-Chang^1,2, QIAO Fei², JIANG Xue-Fei¹, Zhou Shuang-yun³, Ji Fu-sang¹, Li Xin-guo^1,*

¹College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228, China; ²Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China; ³Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Corresponding author: Li Xin-guo; E-mail: lixinguo13@163.com

Abstract:

To study the changes in Ca²⁺-ATPase activities of microsome membrane under salt stress, the Brazilian banana seedlings were treated with the exogenous CaCl₂ and Ca²⁺ chelating agent EGTA under 60 mmol•L^-1 NaCl for 4, 8, 12, 24 and 48 h, and Ca²⁺-ATPase activities of part of cell membrane in leaves and roots were determined. The results showed that Ca²⁺-ATPase activities of plasma membrane, tonoplast membrane and endoplasmic reticulum membrane in roots and leaves were different, and the Ca²⁺-ATPase activity with CaCl₂ treatment was significantly higher than those of other three treatment. Under NaCl stress for 4 h, the Ca²⁺-ATPase activities with CaCl₂ treatment were significantly increased, while the Ca²⁺-ATPase activities with EGTA treatment decreased significantly. The Ca²⁺-ATPase activities of the plasma membrane and tonoplast membrane were much larger than that of the endoplasmic reticulum membrane. These indicated that Ca²⁺-ATPase activity of plasma membrane and tonoplast membrane mainly involved in the regulation of Ca²⁺ homeostasis in Brazilian banana seedlings.

Key words: Brazilian banana seedling; salt stress; Ca²⁺-ATPase activity